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Figure 2 | BMC Research Notes

Figure 2

From: Purification and functional characterization of protoplasts and intact vacuoles from grape cells

Figure 2

Microscopic characterization of intact vacuoles purified from grape protoplasts. Intact vacuoles, purified after protoplast lysis, labelled with the fluorescent membrane marker FM1-43 (A; inset: membranous bodies attached to the central vacuole forming an "impure vacuole") observed under the fluorescence microscope. Intact vacuoles labelled with neutral red in the absence and in the presence of 2.5 mM NH4Cl and 25 μM CCCP (B).

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