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Table 1 Primer sequences, purpose, annealing temperature (TA), number of bands and product size for primers during PCR, accession number and the linkage groups (LG) where the fragments were localized in rainbow trout

From: Clock genes and their genomic distributions in three species of salmonid fishes: Associations with genes regulating sexual maturation and cell cycling

Primer Name Primer Sequence (5'→3')   TA # of Bands Band size Sequence Name (GB Accession #) Family & parent LG Arm
Clock1a F-GTCCGTCTCTGCTATTGAGATG Sequencing 61 2 OmyClock1a    
  R-AGTCACCAGCTTAGTCAGCAAC    1.5 kb, 2.5 kb (GU228520)    
Clock1a-1 F-CCCAGTGTCCAGGTGAGAAA Mapping 57    44♀♂ 8 c
  R-CCTTTGTTCAGGGACACATT        
Clock1a-2 F-ACCCTGGGCATCTTTCTTTT Mapping 57    25♀♂ 8 c
  R-GGAAAACTGTACCATGGGCTA        
Clock1b F-ACCCAGAATACAGGGCAGAAC Sequencing 51 2 OmyClock1b    
  R-CAGGATCAATTGAGTGGACTGG    1.5 kb, 1.3 kb (GU228521)    
Clock1b-1 F-AACATGGCCCCTTCCATT Mapping 60    25♂ 24 p
  R-TCTCACCTGGACACTGGACA      44♀ 24 p
Clock3 F-TTGGAAAGGGTAAATCCTGCT Sequencing 51 2 OmyClock3    
  R-CTCGCACCTCCGCATAACT    660 bp, 426 bp (GU228522)    
Clock3-1 F-CACCAGTGGAACTCCAAGC Mapping 61    25♂ 20 p
  R-CGCACCTCCGCATAACTACA        
NPAS2 F-TGCAGTTTGGAAAGGGTAAA Sequencing 51 2 OmyNPAS2    
  R-GAAGTGGCCATGTCAGGTG    602 bp, 487 bp (GU228523)    
NPAS2-1 F-TTGGAAAGGGTAAATCCTGCT Mapping 61    25♀ 9 p
  R-GCCAGATCTTTCTTCAAGACG        
Amh F-ACTCCTCCATGTGGTGCAA Mapping     25♀♂ 8 c
  R-CCATACAGTCTTATCTGATCATGC      44♀♂   
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BMC Research Notes

ISSN: 1756-0500

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