Clock genes and their genomic distributions in three species of salmonid fishes: Associations with genes regulating sexual maturation and cell cycling

Table 1 Primer sequences, purpose, annealing temperature (TA), number of bands and product size for primers during PCR, accession number and the linkage groups (LG) where the fragments were localized in rainbow trout

Primer Name	Primer Sequence (5'→3')		TA	# of Bands Band size	Sequence Name (GB Accession #)	Family & parent	LG	Arm
Clock1a	F-GTCCGTCTCTGCTATTGAGATG	Sequencing	61	2	OmyClock1a
	R-AGTCACCAGCTTAGTCAGCAAC			1.5 kb, 2.5 kb	(GU228520)
Clock1a-1	F-CCCAGTGTCCAGGTGAGAAA	Mapping	57			44♀♂	8	c
	R-CCTTTGTTCAGGGACACATT
Clock1a-2	F-ACCCTGGGCATCTTTCTTTT	Mapping	57			25♀♂	8	c
	R-GGAAAACTGTACCATGGGCTA
Clock1b	F-ACCCAGAATACAGGGCAGAAC	Sequencing	51	2	OmyClock1b
	R-CAGGATCAATTGAGTGGACTGG			1.5 kb, 1.3 kb	(GU228521)
Clock1b-1	F-AACATGGCCCCTTCCATT	Mapping	60			25♂	24	p
	R-TCTCACCTGGACACTGGACA					44♀	24	p
Clock3	F-TTGGAAAGGGTAAATCCTGCT	Sequencing	51	2	OmyClock3
	R-CTCGCACCTCCGCATAACT			660 bp, 426 bp	(GU228522)
Clock3-1	F-CACCAGTGGAACTCCAAGC	Mapping	61			25♂	20	p
	R-CGCACCTCCGCATAACTACA
NPAS2	F-TGCAGTTTGGAAAGGGTAAA	Sequencing	51	2	OmyNPAS2
	R-GAAGTGGCCATGTCAGGTG			602 bp, 487 bp	(GU228523)
NPAS2-1	F-TTGGAAAGGGTAAATCCTGCT	Mapping	61			25♀	9	p
	R-GCCAGATCTTTCTTCAAGACG
Amh	F-ACTCCTCCATGTGGTGCAA	Mapping				25♀♂	8	c
	R-CCATACAGTCTTATCTGATCATGC					44♀♂

ISSN: 1756-0500