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Table 2 Primer sequences, purpose, annealing temperature (TA), number of bands and product size for primers during PCR, accession number and the linkage groups (LG) where the fragments were localized in Atlantic salmon

From: Clock genes and their genomic distributions in three species of salmonid fishes: Associations with genes regulating sexual maturation and cell cycling

Primer Name

Primer Sequence (5'→3')

 

TA

# of Bands Band size

Sequence Name (GB Accession #)

Family & parent

LG

Arm

Clock1a-1

F-CCCAGTGTCCAGGTGAGAAA

Mapping

57

  

BR5♀

2

qa

 

R-CCTTTGTTCAGGGACACATT

       

Clock1a-2

F-ACCCTGGGCATCTTTCTTTT

Mapping

57

  

BR5♂

2

qa

 

R-GGAAAACTGTACCATGGGCTA

       

Clock1b

F-ACCCAGAATACAGGGCAGAAC

Sequencing

51

2

SsaClock1b

   
 

R-CAGGATCAATTGAGTGGACTGG

  

1.5 kb, 1.3 kb

(GU228525)

   

Clock1b-2

F-GGTGCAGATGTTCCTCCAA

Mapping

57

  

BR6♀

18

q

 

R-TCACAGAAGACTGTGAGAGGAC

       

Clock1b-3

F-GAGTACTGCCCTGCAGGTTG

Mapping

55

  

BR5♀

18

q

 

R-TTGACCATGGCCCTCTTATG

       

NPAS2-2

F-CCTCCTGAGGGGGAGAAAG

Mapping

55

  

BR6♀

5

qb

 

R-GAAGTGGCCATGTCAGGTG

       
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BMC Research Notes

ISSN: 1756-0500

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