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Table 2 Primer sequences, purpose, annealing temperature (TA), number of bands and product size for primers during PCR, accession number and the linkage groups (LG) where the fragments were localized in Atlantic salmon

From: Clock genes and their genomic distributions in three species of salmonid fishes: Associations with genes regulating sexual maturation and cell cycling

Primer Name Primer Sequence (5'→3')   TA # of Bands Band size Sequence Name (GB Accession #) Family & parent LG Arm
Clock1a-1 F-CCCAGTGTCCAGGTGAGAAA Mapping 57    BR5♀ 2 qa
  R-CCTTTGTTCAGGGACACATT        
Clock1a-2 F-ACCCTGGGCATCTTTCTTTT Mapping 57    BR5♂ 2 qa
  R-GGAAAACTGTACCATGGGCTA        
Clock1b F-ACCCAGAATACAGGGCAGAAC Sequencing 51 2 SsaClock1b    
  R-CAGGATCAATTGAGTGGACTGG    1.5 kb, 1.3 kb (GU228525)    
Clock1b-2 F-GGTGCAGATGTTCCTCCAA Mapping 57    BR6♀ 18 q
  R-TCACAGAAGACTGTGAGAGGAC        
Clock1b-3 F-GAGTACTGCCCTGCAGGTTG Mapping 55    BR5♀ 18 q
  R-TTGACCATGGCCCTCTTATG        
NPAS2-2 F-CCTCCTGAGGGGGAGAAAG Mapping 55    BR6♀ 5 qb
  R-GAAGTGGCCATGTCAGGTG