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Table 3 PCR amplifications of mitochondrial and chloroplast genome specific sequences on ten randomly selected clones of the CinS2S2 and CinS1S4 libraries detected after filter hybridization.

From: Construction and characterization of two BAC libraries representing a deep-coverage of the genome of chicory (Cichorium intybus L., Asteraceae)

  Mitochondrial genome specific sequences Chloroplast genome specific sequences
  Clone cox2 atpA atp9 cob Clone matK 2 kb* tnrl-trnf 47 kb* rbcl 54 kb* ndhF 124 kb*
CinS2S2 (90 kb) 06I01 - - - + 02L02 + - - -
  09A06 + + + - 04M20 - - - -
  09E24 - - - - 06I22 + - - +
  09I04 - - - - 12E23 - + - -
  13F07 - - - - 26G24 + + + -
  13M05 - + - - 32H03 - + + +
  26G13 - - - + 37C04 + + - -
  27J01 - - - - 41L23 - + + +
  41H20 + + + - 51H20 - - + -
  54B09 - - - - 61O16 - + + -
CinS1S4 (120 kb) 01H06 + + - - 02N01 - + + +
  16P11 + + - - 21F01 + - - +
  22B14 - - - - 45D02 + + + +
  25G18 - - - + 51D03 - - + +
  27O16 - + + - 55M03 + + + +
  31K22 + + + - 62G03 + + + +
  91G03 - + + - 72G02 - + + +
  92M03 + + + - 84D02 + - - +
  96A09 - + + - 91N03 + + + +
  108A12 - - - - 106P01 + + + +
  1. + indicates the presence of a PCR product of the expected size after agarose gel electrophoresis;
  2. - indicates no amplification; * Sequence coordinates on H. annuus and L. sativa chloroplast genome according to [37].