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Table 3 PCR amplifications of mitochondrial and chloroplast genome specific sequences on ten randomly selected clones of the CinS2S2 and CinS1S4 libraries detected after filter hybridization.

From: Construction and characterization of two BAC libraries representing a deep-coverage of the genome of chicory (Cichorium intybus L., Asteraceae)

 

Mitochondrial genome specific sequences

Chloroplast genome specific sequences

 

Clone

cox2

atpA

atp9

cob

Clone

matK 2 kb*

tnrl-trnf 47 kb*

rbcl 54 kb*

ndhF 124 kb*

CinS2S2 (90 kb)

06I01

-

-

-

+

02L02

+

-

-

-

 

09A06

+

+

+

-

04M20

-

-

-

-

 

09E24

-

-

-

-

06I22

+

-

-

+

 

09I04

-

-

-

-

12E23

-

+

-

-

 

13F07

-

-

-

-

26G24

+

+

+

-

 

13M05

-

+

-

-

32H03

-

+

+

+

 

26G13

-

-

-

+

37C04

+

+

-

-

 

27J01

-

-

-

-

41L23

-

+

+

+

 

41H20

+

+

+

-

51H20

-

-

+

-

 

54B09

-

-

-

-

61O16

-

+

+

-

CinS1S4 (120 kb)

01H06

+

+

-

-

02N01

-

+

+

+

 

16P11

+

+

-

-

21F01

+

-

-

+

 

22B14

-

-

-

-

45D02

+

+

+

+

 

25G18

-

-

-

+

51D03

-

-

+

+

 

27O16

-

+

+

-

55M03

+

+

+

+

 

31K22

+

+

+

-

62G03

+

+

+

+

 

91G03

-

+

+

-

72G02

-

+

+

+

 

92M03

+

+

+

-

84D02

+

-

-

+

 

96A09

-

+

+

-

91N03

+

+

+

+

 

108A12

-

-

-

-

106P01

+

+

+

+

  1. + indicates the presence of a PCR product of the expected size after agarose gel electrophoresis;
  2. - indicates no amplification; * Sequence coordinates on H. annuus and L. sativa chloroplast genome according to [37].