Construction and characterization of two BAC libraries representing a deep-coverage of the genome of chicory (Cichorium intybus L., Asteraceae)

Table 4 Fingerprint analysis of BAC clones containing mt genes.

				Informative fingerprint fragment (kb)
Library	Clone	mt gene present	Insert size (kb)	4.8 4.9	11.2	5.6	11.7 9.0 8.8	7.0	9.4	3.6 4.4
CinS2S2	09A06	cox2, atpA, atp9	80	-	-	+	+	-	-	-
CinS1S4	96A09	atpA, atp9	150	-	-	-	+	+	+	-
CinS1S4	92M03	cox2, atpA, atp9	150	-	-	+	+	+	+	-
CinS1S4	31K22	cox2, atpA, atp9	95	-	+	+	+	+	-	-
CinS2S2	06I01	cob	55	+	+	-	-	-	+	+
CinS2S2	26G13	cob	60	+	+	-	-	-	-	+
CinS1S4	25G18	cob	25	-	-	-	-	-	+	+

DNA of BAC clones was digested with Not I for insert size determination, and with Eco RI for fingerprint analysis. Fingerprint fragments ranging from 3 to 12 kb were analysed, and fragments were considered as informative when present in at least 2 clones. Only the results for clones containing at least atpA and atp9, and sharing a fragment with cob-containing clones are shown. Clone 09A06 was included as it contained the smallest insert with the 3 genes atpA, atp9, and cox2. + indicates presence of a fingerprint fragment; - indicates absence of a fingerprint fragment.

ISSN: 1756-0500