Figure 2

Long non-protein coding RNAs are deregulated during ATRA differentiation of NB4 cells. A) In the table are showed the 58 ncRNAs found deregulated in ATRA treated NB4 cells. Asterisks indicate the ncRNAs validate by quantitative real-time PCR. B) Real time confirmed the microarray data. The experiments, performed three times, are shown as fold induction of ATRA NB4 vs. untreated NB4 (UT). The bars indicates ± SEM. Statistical analysis was performed with Prism v4.0 (GraphPad software); asterisk indicate significant differences between ATRA treated NB4 and UT NB4 according to the two-tailed unpaired Student's t test (*, P < 0.05). The primer sequences, used for the real time PCR are shown in the supplementary file 3.