Figure 1
Yeast two-hybrid assay. Yeast strain AH109 cotransformed with pGBKT7-CB or pGBKT7-CBSH3- bait plasmids and pGADT7-eIF3H prey plasmid grew on SD/-Leu/-Trp/-Ade/-His/X-α-Gal/5 mM 3AT plates, whereas a control transformant, with a pGBKT7 empty vector and pGADT7-eIF3H, did not. As CB-bait specific positive interaction control, yeast strain AH109 was cotransformed with pGBKT7-CB or pGBKT7-CBSH3- and pGADT7-gephyrin vectors. Results shown are representative of at least three independent experiments.