Figure 2
From: Selective amplification of Brucella melitensis mRNA from a mixed host-pathogen total RNA
Integrity and composition of the host: Brucella RNA samples pre- and post-treatment. Twenty-five (25) μg total eukaryote RNA from human (HeLa S3) and bovine (MDBK) cell lines were mixed with 2 μg of Brucella melitensis 16 M RNA (ratio 12.5:1) and treated with MICROB Enrich™(Ambion) according to the instruction manual. (A) Agarose gel electrophoresis image. Lines 1 & 2: HeLa cell line:B.melitensis 16 M RNA mix; lines 3 & 4: MDBK cell line:B.melitensis 16 M RNA mix; pre and post-treatment respectively. (B) Comparison of RNA composition from a sample of MDBK cell line:B. melitensis 16 M pre and post-treatment with MICROB Enrich™ (Ambion) and examined on an Agilent 2100 Bioanalyzer. Ribosomal RNA subunits are indicated.