PCR assay for genome specificity of TaRAD51 and TaDMC1 cDNA homoeologues in bread wheat. Each primer set for TaRAD51 was used to amplify NT7A7B, NT7A7D, NT7B7A, NT7B7D, NT7D7A, NT7D7B, water control and CS (a) TaRAD51(A), (B) & (D) genome-specific primer PCR amplification. Each primer set for TaDMC1 was used to amplify NT5A5B, NT5A5D, NT5B5A, NT5B5D, NT5D5A, NT5D5B, water control and CS.(b) TaDMC1(A), (B) & (D) genome-specific primer PCR amplification. Absence of bands in the group 7 & 5 Nullisomics allocates the primer to the respective genomes; M = 2-log ladder (NEB).