Table 1 List of primers used in the present study

OsRAD51 Exon 1-2-F

CCTCACATCCCGAGCATCTC

60

171

Cloning of full-length cDNA of wheat RAD51

OsRAD51 Exon 1-2-R

TCACATCAACTGCAGCTATTCCAG

OsRAD51 Exon 8-9-F

GGTAGTGGCCCAAGTGGATG

60

564

OsRAD51 Exon 8-9-R

TGGTGGTCCAATATCACATAGGAG

OsRAD51 Exon 1-7-F

ATGTCGTCGGCGGCGG

65

889

OsRAD51 Exon 1-7-R

CTCATCCGCTAATTTCTGAAGGC

OsRAD51 Exon 2-9-F

AAGTCGATAAGATAAT TGAAGCA

60

879

OsRAD51 Exon 2-9-R

CTGAAACCTTGCTTCAGCT

NotI-(dT)₁₈

AACTGGAAGAATTCGCGGCCGCAGGAA(T)₁₈

Cloning of full-length cDNA of wheat DMC1

Not I(dT) anchor

AACTGGAAGAATTCGCGG

TaDMC1 -DGSP1

GAAGAGCCTTTCAGGCTTCTG

55

700

TaDMC1- DGSP2

GGTGAACTTGCAGAGCGCCAG

55

772

TaDMC1- DGSP3

CTGGCGCTCTGCAAGTTCACC

55

772

TaDMC1- DGSP4

GGAAGACCCAGTTGGCTCAT

55

461

TaDMC1- DGSP5

GGTGAACTTGCAGAGCGTCAG

55

207

TaRAD51 Exon 8-F

CCATGATGGTGGAGACAAGG

65

2500

Amplification of intronic regions in TaRAD51 &TaDMC1 genes

TaRAD51 3'UTR-R

TGGTGGTCCAATATCACATAGGAG

TaDMC1-5'UTR-F

ATGATCCACATTCCACCCGC

65

2500

TaDMC1-Exon 6-R

ATGAGCCAACTGGGTCTTCC

iTaRAD51-7A&7D-F

CGGAAGGATTGGTA AAAAAT

55

450 & 500

TaRAD51 &TaDMC1 genome-specific primers based on intronic regions for localization on wheat genome

iTaRAD51-7A&7D-R

CACTCAGAAATGACG AAAAAGG

iTaRAD51-7B-F

ATGGTGGAGACAA GGTGAG

63

500

iTaRAD51-7B-R

GTACACTAGCATTAC GGTACAG

iTaDMC1-5A-F

AGCCTCCGCCCCACTTCCTTC

63

500

iTaDMC1-5A-R

ACAAACGCAACACGA GCACACG

iTaDMC1-5B-F

AGCCTTGGCCCCACTTCCTC

63

500

iTaDMC1-5B-R

ACGCGCTGCACGCA CCAAA

iTaDMC1-5D-F

CTCCTCTGACGCAG GCGGA

63

389

iTaDMC1-5D-R

ACGCGCTGCACGC ACCAAA

TaRAD51-UTR-F

CCTCACATCCCGAGCATCTC

63

1528

TaRAD51 cDNA full-length primers including UTR's

TaRAD51-UTR-R

CTCCTATGTGATATTGGACCACCA

eTaRAD51-7A-F

GGGGATACCTCGTGTATCAGACT

58

328

TaRAD51 genome-specific primers based on exons for QRT-PCR analyses

eTaRAD51-R

TGGTGGTCCAATATCACATAGGAG

eTaRAD51-7B-F

GATACATCGTGTATCGGACA

58

328

eTaRAD51- R

TGGTGGTCCAATATCACATAGGAG

eTaRAD51-7D-F

TGGGGATACATCGTGTATTGGCCT

65

327

eTaRAD51- R

TGGTGGTCCAATATCACATAGGAG

TaDMC1-UTR-F

ATGATCCACATTCCACCCGC

63

1268

TaDMC1 cDNA full-length primers including UTR's

TaDMC1-UTR-R

CACTGCAGAAAAGAAATTGGGCAAC

eTaDMC1-5A-F

AGCCTCCGCCCCACTTCCTTC

65

150

TaDMC1 genome-specific primers based on exons QRT-PCR analyses

eTaDMC1-R

CTTGTCGATGGACTCGAAGCACTC

eTaDMC1-5B-F

AGCCTTGGCCCCACTTC CTC

65

150

eTaDMC1-R

CTTGTCGATGGACTCGAAGCACTC

eTaDMC1-5D-F

TTCTCCTCCAGCAGCACGCGAA

65

150

eTaDMC1-R

CTTGTCGATGGACTCGAAGCACTC

TUB-F

TCTTCATGGTGGGCTTCGC

55

475

QRT-PCR control primers

TUB-R

CGCCTCGGGTGAACTCCATCT