Alternative
transcript
|
Donor splice
site location
(nt)a
|
Acceptor
splice site
location
(nt)a
|
Intron size
/kb
|
EXON/intron/EXON
(consensus splice sequence)b
5' CAG|guragu...cu/gac/u(y)10 15nyag|G) 3'
|
---|
CHD7
CRA_e
|
Exon 3 (47)
|
Exon 36 (112)
|
81
|
5' CAG|gu uagu...c agau gugcuguuuuccuauuucag|A 3'
|
- a) Location of the exonics donor and acceptor splice sites of the CHD7 CRA_e alternative transcript. The numbers in parenthesis represent the exonic splicing sites at exons 3 and 36; b) The consensus sequence associated to splice donor, branch and acceptor sites according to Padgett [30] and Keller and Noon [31]. The vertical lines represent either 5' or 3' splice site and the putative branch point is underlined. The sequence of the investigated splice site in the CHD7 CRA_e transcript is represented below the consensus splice sequence. Nucleotides identity between the consensus and the investigated splice sites are shown in bold r = purine (A or G); y = pyrimidine (C or U)