| Two-tier Serology | Total |
---|
 | + | - |  |
---|
Nested PCR + | 1 | 6 | 7 |
Nested PCR - | 1 | 122 | 123 |
Total | 2 | 128 | 130 |
- + = positive
- - = negative
- Laboratory detection of Lyme disease among 130 ER/walkin patients:
- Confirmed case prevalence = (7+1)/130 = 8/130 = 6.2% (DNA sequencing or 2-tier serology)
- Sensitivity of nested PCR = 87.5% (7/8)
- Sensitivity of 2-tier seropositivity = 25% (2/8)