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Table 2 Comparison of nested PCR and 2-tier serology in detection of Lyme disease among 130 patients visiting emergency room and walk-in clinic

From: Early Lyme disease with spirochetemia - diagnosed by DNA sequencing

  Two-tier Serology Total
  + -  
Nested PCR + 1 6 7
Nested PCR - 1 122 123
Total 2 128 130
  1. + = positive
  2. - = negative
  3. Laboratory detection of Lyme disease among 130 ER/walkin patients:
  4. Confirmed case prevalence = (7+1)/130 = 8/130 = 6.2% (DNA sequencing or 2-tier serology)
  5. Sensitivity of nested PCR = 87.5% (7/8)
  6. Sensitivity of 2-tier seropositivity = 25% (2/8)