A ranking index for quality assessment of forensic DNA profiles

BMC Research Notes

Table 2 Electropherogram data for the DNA profile in Figure 1A

Locus	Allele 1	Peak height (rfu)	Allele 2	Peak height (rfu)	TPH	MLB	SH	FI
D3S1358	14	244	17	201
vWA	15	165	17	226
D16S539	12	146	13	115
D2S1338	17	79
D8S1179	14	174	15	240	2628	0.81	2.14	0.94
D21S11	32.2	177	33.2	113
D18S51	16	61	19	61
D19S433	14	416
TH01	9	123
FGA	21	87	d.o	d.o

d.o: drop-out allele
The DNA profile was obtained by analysing a swab of a spoon found in a honey jar, DNA concentration 0.09 ng/μl, using the standard DNA polymerase AmpliTaq Gold and AmpFl STR SGM Plus primers. The STR markers D2S1338, D19S433 and TH01 are homozygous (one allele expected), the other markers are heterozygous (two alleles expected). Peak heights are given in relative fluorescence units (rfu). Total sum of Peak Heights (TPH), Mean Local Balance (MLB), Shannon entropy (SH) and the calculated forensic DNA profile index (FI) are presented.

ISSN: 1756-0500