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Table 1 Number of reads eliminated at different steps of the tsfs.pl module

From: CANGS: a user-friendly utility for processing and analyzing 454 GS-FLX data in biodiversity studies

Order of steps Steps Total no. of sequences No. of sequences considered No. of sequences discarded
1 Removal of Adapter B 447,909 373,116 74,793
2 Filtering sequences with ambiguities 373,116 357,926 15,190
3 Removal of singletons 357,926 311,425 46,501
4 Grouping of sequences according to bar codes 311,425 306,042 5,383
5 Filtering sequences according to length threshold 306,042 305,884 158
6 Removal of PCR primers 305,884 282,053 23,831
7 Quality filtering 282,053 281,003 1,050
  Total Sequences 447,909 281,003 166,906