From: Temporal reliability of cytokines and growth factors in EDTA plasma
Biomarker | LLD | First Sample | Second Sample | Intra-batch CV | ICC | (95% CI) | ||||
---|---|---|---|---|---|---|---|---|---|---|
% Above LLD | Median | (25%, 75%) | % Above LLD | Median | (25%, 75%) | |||||
hsIL-1β (pg/mL)b | 0.06 | 100 | 8.1 | (5.5, 11.3) | 100 | 8.0 | (4.6, 10.1) | 2.0% | 0.73 | (0.43-0.89) |
hsIL-2 (pg/mL)b | 0.16 | 100 | 28.8 | (18.9, 39.1) | 100 | 31.3 | (20.8, 39.9) | 2.7% | 0.80 | (0.56-0.92) |
sIL-2R (pg/ml)c | 30 | 100 | 652 | (624, 789) | 100 | 691 | (624, 762) | 1.0% | 0.86 | (0.68-0.95) |
IL-4 (pg/mL)c | 5 | 100 | 140 | (114, 163) | 100 | 120 | (109, 143) | 1.6% | 0.00 | (-0.45-0.45) |
hsIL-4 (pg/mL)b | 0.13 | 100 | 50.5 | (31.4, 80.7) | 100 | 42.4 | (16.2, 83.0) | 2.7% | 0.70 | (0.36-0.87) |
hsIL-5 (pg/mL)b | 0.01 | 100 | 3.4 | (1.8, 4.6) | 100 | 3.2 | (1.8, 4.2) | 2.5% | 0.73 | (0.41-0.89) |
hsIL-6 (pg/mL)b | 0.10 | 100 | 20.4 | (15.9, 28.7) | 100 | 18.4 | (13.8, 24.3) | 2.0% | 0.81 | (0.56-0.92) |
sIL-6R (ng/mL)c | 0.024 | 100 | 29.0 | (26.5, 42.6) | 100 | 29.8 | (25.2, 36.4) | 0.3% | 0.69 | (0.36-0.87) |
hsIL-7 (pg/mL)b | 0.12 | 100 | 10.1 | (6.5, 15.7) | 94.4 | 10.7 | (6.1, 13.7) | 3.3% | 0.55 | (0.14-0.80) |
hsIL-8 (pg/mL)b | 0.11 | 100 | 9.3 | (6.4, 11.8) | 100 | 8.3 | (5.3, 10.5) | 1.6% | 0.86 | (0.68-0.95) |
hsIL-10 (pg/mL)b | 0.15 | 100 | 40.7 | (26.7, 57.2) | 100 | 33.2 | (15.8, 56.3) | 2.1% | 0.75 | (0.46-0.90) |
IL-12p40 (pg/mL)c | 15 | 100 | 410 | (379, 471) | 100 | 413 | (402, 447) | 0.7% | 0.89 | (0.73-0.96) |
hsIL-12p70 (pg/mL)b | 0.11 | 100 | 32.3 | (21.5, 46.5) | 100 | 26.0 | (11.8, 40.7) | 2.1% | 0.77 | (0.50-0.91) |
hsIL-13 (pg/mL)b | 0.48 | 100 | 45.7 | (30.5, 51.9) | 100 | 39.6 | (15.4, 50.2) | 3.0% | 0.81 | (0.56-0.92) |
CRP (μg/mL)b | 0.000002 | 100 | 10.6 | (3.1, 17.1) | 100 | 8.5 | (4.0, 16.9) | 1.9% | 0.76 | (0.48-0.90) |
IFNα (pg/mL)c | 15 | 100 | 45.8 | (41.1, 70.7) | 100 | 50.2 | (37.8, 80.7) | 5.1% | 0.53 | (0.11-0.79) |
IFNγ (pg/mL)c | 5 | 100 | 175 | (108, 199) | 100 | 118 | (95.3, 162) | 1.8% | 0.00 | (-0.45-0.45) |
hsIFNγ (pg/mL)b | 0.29 | 100 | 78.3 | (42.6, 99.4) | 100 | 65.0 | (43.4, 103) | 2.2% | 0.72 | (0.41-0.89) |
hsTNFα (pg/mL)b | 0.05 | 100 | 8.0 | (6.2, 11.2) | 100 | 8.4 | (5.8, 10.9) | 1.9% | 0.69 | (0.36-0.87) |
sTNF-R1 (ng/mL)c | 0.015 | 100 | 1.3 | (1.2, 1.8) | 100 | 1.2 | (0.9, 1.6) | 1.7% | 0.31 | (-0.16-0.67) |
sTNF-R2 (ng/mL)c | 0.015 | 100 | 1.1 | (0.72, 1.2) | 100 | 0.9 | (0.7, 1.1) | 0.5% | 0.68 | (0.33-0.86) |
Growth Factors | ||||||||||
EGFR (ng/mL)d | 0.020 | 100 | 15.4 | (14.1, 15.9) | 100 | 15.3 | (13.9, 16.6) | 0.5% | 0.93 | (0.83-0.97) |
ErbB2 (ng/mL)d | 0.017 | 100 | 3.4 | (2.9, 3.6) | 100 | 3.2 | (3.0, 3.8) | 0.5% | 0.63 | (0.26-0.84) |
hsGM-CSF (pg/mL)b | 0.46 | 100 | 38.2 | (29.6, 52.4) | 100 | 33.5 | (21.9, 39.9) | 2.9% | 0.47 | (0.03-0.76) |
G-CSF (pg/mL)c | 15 | 100 | 129 | (108, 167) | 100 | 129 | (108, 160) | 6.4% | 0.75 | (0.46-0.90) |
HGF (pg/mL)c | 10 | 100 | 151 | (101, 186) | 100 | 196 | (101, 278) | 1.1% | 0.74 | (0.43-0.89) |