Typical growth pattern of MNNG-HOS cells grafted to the CAM. A - C: In vivo microscopy (10×, scale bar 1 mm) documenting the typical growth pattern after implantation of MNNG-HOS cells, arrowheads indicate tumour boundaries. A 1 day after grafting. After 4 days a vascularized tumour becomes apparent (B). Solidification of the tumour steadily progresses until day 7 (C). Note the bleeding caused by the tumour in C. D (12.5×, scale bar 0.8 mm) and E (50×, scale bar 20 μm) are higher magnifications of C after turning the CAM upside down. Note the three smaller tumour nodules that were not visible from the upside (C). F (12.5×, scale bar 0.8 mm) and G (50×, scale bar 20 μm) is another example of a typical MNNG-HOS tumour. Note the rich vascularization of the tumour (G).