Comparison of different methods for preparation and characterization of total RNA from cartilage samples to uncover osteoarthritis in vivo

Table 2 Recommendation for the use of different methods for RNA isolation from cartilage/chondrocytes.

METHOD	MATERIAL
	chondrocytes	comments	cartilage	comments
Trizol^®	++	solid RNA quality	-	RNA degradation
		high RNA yield
RNeasy™	+++	high RNA quality	-	RNA degradation
		solid RNA yield
Trizol^®/RNeasy™	+	cost-benefit ratio (-)	+/++	solid RNA quality
		solid RNA quality		(difficulties with human samples)
		solid RNA yield		solid RNA yield
RNAqueous™	+	cost-benefit ratio (-)	+++	high RNA quality
		solid RNA quality		solid RNA yield
		solid RNA yield

The results/criteria for recommendation are given as follows: - fail; + passing; ++ good; +++ very good.

ISSN: 1756-0500