Figure 1

The dye labeled size standards for molecular mass determination. A) IR dye labeled size standards for Li-COR Analyzers. Comparison of custom generated size standards with the commercially available size standard from Li-COR Biosciences. The numbers on left and right side of gel picture refer to size of fragments in bp respectively. Lane 1 - Custom generated size standards, Lane 2 - Li-COR size standards. B) Customized size standards used for SNP detection. Images of Li-COR gel showing resolved size standards in 700 and 800 channels. One of the samples having multiple SNPs (demarcated as black circles in the image) is shown. The samples were amplified with IR dye labeled gene specific primers for LeACS-2. A full-length fragment of 928 bases was obtained. PCR amplification, CEL I digestion and electrophoresis on polyacrylamide gel was done as previously described by Till et. al., 2006 [2]. Lanes 1, 2, 3, 4, 5 - DNA samples from different tomato accessions doped with reference DNA (Arka Vikas); Lane M - customized size standards.