Figure 1
The influence of freeze/thaw cycles on tHDA enzymatic activity. Unencapsulated reaction mixtures were subjected to either 0 (lane 2), 5 (lane 3), 10 (lane 4), or 20 (lane 5) cycles of freeze-thawing. Lane 1 is a 50 bp DNA ladder. The 1350 bp, 100 bp, and 50 bp bands are labeled. Reaction products were visualized by ethidium bromide staining of a 1.8% agarose gel. The full length reaction product is 85 bp.