Figure 2

The effect of temperature on tHDA enzymatic activity. (A) The influence of temperature on the enzymatic activity of the isothermal tHDA system. Samples were incubated at 65°C (lane 2), 37°C (lane 3), 23°C (lane 4), and 4°C (lane 5) for 1.5 h. Lane 1 contains a 50 bp DNA ladder. The 1350 bp, 100 bp, and 50 bp bands are labeled. (B) The influence of overnight incubation temperature on the enzymatic activity of the isothermal tHDA system. Samples were incubated at 4°C (lane 2) and 23°C (lane 3) overnight prior to incubation at 65°C for 1.5 h. Lane 1 is a 50 bp DNA ladder. Reaction products were observed by ethidium bromide staining of a 1.8% agarose gel.