Figure 3

In silico promoter analysis and genomic organization. (A) Statistically overrepresented (p < 0.05) potential transcription factor binding sites (TFBS) identified by in silico analysis of genomic regions from -3 kb to + 2 kb around transcription start sites of genes with increasing gene expression at late stages of adipocyte differentiation (cluster F) using position frequency (weight) matrices from TRANSFAC and JASPAR. Corresponding sequence logos are shown and number of targets within the dataset (cluster) is given and tested against the number of targets from all RefSeq transcripts using Fishers exact test. Note only transcription factor motifs are considered which are exclusively overrepresented in cluster F. (B) The genomic organization around Foxo1 using the UCSC genome browser (NCBI37/mm9) with high similarity to the Pparg-Rxra DR-1 motif (similarity score > 0.90) (blue '+' strand, red '-'strand), Pparg binding regions as identified by previous studies at day 6 [42] (dark red) and day 10 [43] (orange) of 3T3-L1 adipocyte differentiation.