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Table 4 Ranking order of candidate reference genes in the intervertebral disc of Sprague-Dawley (SD) rat and New Zealand White (NZW) rabbit

From: Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the rat and rabbit intervertebral disc

 

NormFinder

BestKeeper

Rank

SD rat

NZW rabbit

rat & rabbit

SD rat

NZW rabbit

rat & rabbit

1

Hprt1

(0.541)

CycA

(0.227)

Hprt1

(0.514)

Hprt1

0.35)

Hprt1

(0.23)

Hprt1

(0.30)

2

18S rRNA

(0.755)

Hprt1

(0.371)

18S rRNA

(0.596)

GAPDH

(0.66)

CycA

(0.36)

CycA

(0.68)

3

CycA

(0.788)

Pgk1

(0.374)

CycA

(0.636)

CycA

(0.69)

Pgk1

(0.43)

GAPDH

(0.85)

4

GAPDH

(0.931)

Actb

(0.478)

Actb

(0.658)

18S rRNA

(1.05)

Actb

(0.53)

Actb

(0.97)

5

Actb

(0.947)

Ywhaz

(0.497)

Ywhaz

(0.783)

Actb

(1.23)

Ywhaz

(0.64)

Pgk1

(1.01)

6

Pgk1

(1.417)

18S rRNA

(0.511)

Pgk1

(0.808)

Ywhaz

(2.22)

18S rRNA

(0.90)

Ywhaz

(1.12)

7

Ywhaz

(1.482)

GAPDH

(0.699)

GAPDH

(0.956)

Pgk1

(2.25)

GAPDH

(1.12)

18S rRNA

(1.23)

  1. (value); stability in NormFinder and standard deviation in BestKeeper