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Table 4 Ranking order of candidate reference genes in the intervertebral disc of Sprague-Dawley (SD) rat and New Zealand White (NZW) rabbit

From: Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the rat and rabbit intervertebral disc

  NormFinder BestKeeper
Rank SD rat NZW rabbit rat & rabbit SD rat NZW rabbit rat & rabbit
1 Hprt1
(0.541)
CycA
(0.227)
Hprt1
(0.514)
Hprt1
0.35)
Hprt1
(0.23)
Hprt1
(0.30)
2 18S rRNA
(0.755)
Hprt1
(0.371)
18S rRNA
(0.596)
GAPDH
(0.66)
CycA
(0.36)
CycA
(0.68)
3 CycA
(0.788)
Pgk1
(0.374)
CycA
(0.636)
CycA
(0.69)
Pgk1
(0.43)
GAPDH
(0.85)
4 GAPDH
(0.931)
Actb
(0.478)
Actb
(0.658)
18S rRNA
(1.05)
Actb
(0.53)
Actb
(0.97)
5 Actb
(0.947)
Ywhaz
(0.497)
Ywhaz
(0.783)
Actb
(1.23)
Ywhaz
(0.64)
Pgk1
(1.01)
6 Pgk1
(1.417)
18S rRNA
(0.511)
Pgk1
(0.808)
Ywhaz
(2.22)
18S rRNA
(0.90)
Ywhaz
(1.12)
7 Ywhaz
(1.482)
GAPDH
(0.699)
GAPDH
(0.956)
Pgk1
(2.25)
GAPDH
(1.12)
18S rRNA
(1.23)
  1. (value); stability in NormFinder and standard deviation in BestKeeper