Skip to main content

Table 5 Overall ranking order of candidate reference genes based on the output values from three programs and selection of the best genes in the intervertebral disc of Sprague-Dawley (SD) rat and New Zealand White (NZW) rabbit

From: Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the rat and rabbit intervertebral disc

SD rat Actb GAPDH 18S rRAN CycA Hprt1 Ywhaz Pgk1 Best genes
geNorm 1.515 0.498 1.268 0.498 0.664 2.040 2.406  
NormFinder 0.947 0.931 0.755 0.788 0.541 1.482 1.417 Hprt1 and CycA
BestKeeper 1.23 0.66 1.05 0.69 0.35 2.22 2.25  
Average 1.23 0.70 1.02 0.66 0.52 1.91 2.02  
Rank 5 3 4 2 1 6 7  
NZW rat Actb GAPDH 18S rRAN CycA Hprt1 Ywhaz Pgk1 Best genes
geNorm 0.414 0.903 0.803 0.484 0.565 0.414 0.698  
NormFinder 0.478 0.699 0.511 0.227 0.371 0.497 0.374  
BestKeeper 0.53 1.12 0.90 0.36 0.23 0.64 0.43 CycA Hprt1 and Actb
Average 0.47 0.91 0.74 0.36 0.39 0.52 0.50  
Rank 3 7 6 1 2 5 4  
rat & rabbit Actb GAPDH 18S rRAN CycA Hprt1 Ywhaz Pgk1 Best genes
geNorm 1.427 0.854 1.217 0.598 0.598 2.081 1.804  
NormFinder 0.658 0.956 0.596 0.636 0.514 0.783 0.808  
BestKeeper 0.97 0.85 1.23 0.68 0.30 1.12 1.01 Hprt1 and CycA
Average 1.02 0.89 1.01 0.64 0.47 1.33 1.21  
Rank 5 3 4 2 1 7 6