Table 1 Primers, probes, siRNAs used
From: Increased expression of Ero1L-alpha in healing fetal wounds
|
Primer Number/Name (from Figure 2A, if applicable) | Direction | Primer Sequence |
|---|---|---|
| 1 | F | TCAGCCAGTGTGGAAGGAGGGA |
| 2 | F | AGGAGACGCAGAAGGCTGTTC |
| 3 | F | TTGGGTTGTTTTGGTGGTAGAAAGGT |
| 4 | F | GCCGACAGTCAGCAAGTTTGCTTTATC |
| 5 | F | GCAGGGCTTGTGGATGTAATGTG |
| 6 | R | CTGATGTCATTCCAGAAAGGAC |
| 7 | R | TAACAGCACAGTCCCTCCTTCCACAC |
| 8 | R | TCAGAGCAGTACCCAAACCCTG |
| 9 | R | CGGAACAGCAATGGAGTTGGTAAG |
| clone11 siRNA sense | rGrUrGrCrUrArCrArCrArGrArCrCrUrGrUrCrUTT | |
| clone11 siRNA antisense | rArGrArCrArGrGrUrCrUrGrUrGrUrArGrCrArCTT | |
| scrambled siRNA sense | rUrGrCrGrArUrArCrGrArCrArUrCrCrUrCrGrUTT | |
| scrambled siRNA antisense | rArGrUrArCrCrUrGrCrUrGrGrGrUrCrArGrArATT | |
| Ero1LA real time for | F | ACCTGAAGAGGCCTTGTCCTTT |
| Ero1LA real time rev | R | TCCATCAGGAACTTCATCAGATTG |
| Ero1LA real time probe | P | TGGAATGACATCAGCCAGTGTGGAAG |
| clone11 real time for | F | CTCTGAAAACATGACTCCCTCCTT |
| clone11 real time rev | R | AGGAGTCTGGCTTTCTCCTGAA |
| clone11 real time probe | P | CACCGCTCTGTGACCTCCTGAAC |
| GAPDH real time for | F | CTCTGAAAACATGACTCCCTCCTT |
| GAPDH real time rev | R | CCTCGGTGTAGCCCAGGAT |
| GAPDH real time probe | P | AAGCAGGCATCCGAGGGCCC |