Table 1 Primers, probes, siRNAs used
From: Increased expression of Ero1L-alpha in healing fetal wounds
Primer Number/Name (from Figure 2A, if applicable) | Direction | Primer Sequence |
|---|---|---|
1 | F | TCAGCCAGTGTGGAAGGAGGGA |
2 | F | AGGAGACGCAGAAGGCTGTTC |
3 | F | TTGGGTTGTTTTGGTGGTAGAAAGGT |
4 | F | GCCGACAGTCAGCAAGTTTGCTTTATC |
5 | F | GCAGGGCTTGTGGATGTAATGTG |
6 | R | CTGATGTCATTCCAGAAAGGAC |
7 | R | TAACAGCACAGTCCCTCCTTCCACAC |
8 | R | TCAGAGCAGTACCCAAACCCTG |
9 | R | CGGAACAGCAATGGAGTTGGTAAG |
clone11 siRNA sense | Â | rGrUrGrCrUrArCrArCrArGrArCrCrUrGrUrCrUTT |
clone11 siRNA antisense | Â | rArGrArCrArGrGrUrCrUrGrUrGrUrArGrCrArCTT |
scrambled siRNA sense | Â | rUrGrCrGrArUrArCrGrArCrArUrCrCrUrCrGrUTT |
scrambled siRNA antisense | Â | rArGrUrArCrCrUrGrCrUrGrGrGrUrCrArGrArATT |
Ero1LA real time for | F | ACCTGAAGAGGCCTTGTCCTTT |
Ero1LA real time rev | R | TCCATCAGGAACTTCATCAGATTG |
Ero1LA real time probe | P | TGGAATGACATCAGCCAGTGTGGAAG |
clone11 real time for | F | CTCTGAAAACATGACTCCCTCCTT |
clone11 real time rev | R | AGGAGTCTGGCTTTCTCCTGAA |
clone11 real time probe | P | CACCGCTCTGTGACCTCCTGAAC |
GAPDH real time for | F | CTCTGAAAACATGACTCCCTCCTT |
GAPDH real time rev | R | CCTCGGTGTAGCCCAGGAT |
GAPDH real time probe | P | AAGCAGGCATCCGAGGGCCC |