Figure 3

Stable pooled adherent BHK21 studies. Adherent BHK21 cells were transfected with different plasmids, cultured under hygromycin selection for one month and the levels of BDD mRNA (A), secreted protein (B) and secreted activity (C) were measured. The relative copies of integrated BDD cDNA was measured by qPCR (D). Data was statistically analyzed using 't' test to detect significant differences between the 3.2 Kb and the 1.5 Kb UCOE containing expression constructs (*: p < 0.05 or more).