RT-MLPA assays of M. marinum -infected zebrafish adults and S. typhimurium -infected zebrafish embryos. (A). Zebrafish were infected with M. marinum strains E11 and Mma20, or injected with PBS as a control, and samples were taken at 6 dpi . (B) One-day-old zebrafish embryos (27 hpf) were intravenously infected with S. typhimurium strain SL1027, or injected with PBS as a control, and samples were taken at 8 hpi (35 hpf). Three biological replicates were analyzed in both experiments. A representative example of the RT-MLPA assay result of each is shown. Peak patterns of the FAM-labelled amplification products are in blue and peak patterns of the TR-labelled amplification products are in red. Fold change values of amplification products that were more than 2-fold up-regulated in infected zebrafish compared to the uninfected control are indicated with green arrows, and fold change values of amplification products that were more than 2-fold down-regulated are indicated with purple arrows. Fold change values are based on the combined data from three biological replicates.