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Table 1 Target genes of the zebrafish immune response RT-MLPA assay

From: Rapid screening of innate immune gene expression in zebrafish using reverse transcription - multiplex ligation-dependent probe amplification

Gene name1 Description ZFIN ID GenBank Accession Entrez Gene ID UniGene ID2
Immune response genes     
ccl20a chemokine ccl-c24a (si:dkey-150o13.1, ccl20) ZDB-GENE-091204-95 NM_001113595 563152 Dr.133624
ccl-c24i transcribed locus, weakly similar to NP_001108573.1 chemokine CCL-Cub - CN021049 - Dr.125570
ccl-c5a chemokine ccl-c5a (si:ch211-89f7.4) ZDB-GENE-060526-181 NM_001082906 794891 Dr.133987
chia.6 chitinase, acidic.6 ZDB-GENE-030131-1140 NM_199603 322420 Dr.77223
cxc46 cxc chemokine 46 ZDB-GENE-090303-1 XM_002664187 100321314 Dr.117585
cxcl-c13d chemokine cxcl-c13d - NM_001113651 100003911 Dr.92011
cxcl-c1c chemokine cxcl-c1c (si:ch73-6k14.1) ZDB-GENE-090313-165 NM_001115060 795785 Dr.113696
cxcl-c5c chemokine cxcl-c5c - NM_001115055 567537 Dr.111760
cxcr3.2 chemokine (C-X-C motif), receptor 3.2 ZDB-GENE-041114-186 NM_001007314 791973 Dr.82754
cxcr4b chemokine (C-X-C motif), receptor 4b ZDB-GENE-010614-1 NM_131834 114447 Dr.75485
fkbp5 FK506 binding protein 5 ZDB-GENE-030616-630 NM_213149 368924 Dr.78793
fos v-fos FBJ murine osteosarcoma viral oncogene homolog ZDB-GENE-031222-4 NM_205569 394198 Dr.12986
ifnphi1 interferon phi 1 ZDB-GENE-030721-3 NM_207640 360134 Dr.85981
il10 interleukin 10 ZDB-GENE-051111-1 NM_001020785 553957 Dr.135567
il1b interleukin 1, beta ZDB-GENE-040702-2 NM_212844 405770 Dr.30443
il8 interleukin 8 ZDB-GENE-081104-317 CT826376 100002946 Dr.112992
jun v-jun sarcoma virus 17 oncogene homolog (c-Jun) ZDB-GENE-030131-7859 NM_199987 335916 Dr.1064
junb jun B proto-oncogene ZDB-GENE-040426-2172 NM_213556 407086 Dr.10326
mmp9 matrix metalloproteinase 9 ZDB-GENE-040426-2132 NM_213123 406397 Dr.76275
mpx myeloid-specific peroxidase ZDB-GENE-030131-9460 NM_212779 337514 Dr.75725
myd88 myeloid differentiation primary response gene (88) ZDB-GENE-040219-3 NM_212814 403145 Dr.134592
nfkb2 nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, p49/p100 ZDB-GENE-030131-6701 NM_001001840 415100 Dr.117553
rel v-rel reticuloendotheliosis viral oncogene homolog ZDB-GENE-040718-255 NM_001001841 415101 Dr.86023
rela v-rel reticuloendotheliosis viral oncogene homolog A ZDB-GENE-040825-4 NM_001001839 415099 Dr.84126
sarm1 sterile alpha and TIR motif containing 1 ZDB-GENE-040219-1 NM_001130596 403143 Dr.84799
spi1 spleen focus forming virus (SFFV) proviral integration oncogene spi1 (pu.1) ZDB-GENE-980526-164 NM_198062 30117 Dr.34508
ticam1 toll-like receptor adaptor molecule 1 (ticam1, trif) ZDB-GENE-040219-2 NM_001044759 403147 Dr.82215
tirap toll-interleukin 1 receptor (TIR) domain containing adaptor protein (tirap, mal) ZDB-GENE-040219-4 AL916644 403148 Dr.87438
tlr1 toll-like receptor 1 ZDB-GENE-040220-1 NM_001130593 403127 Dr.89709
tlr4bb toll-like receptor 4b, duplicate b ZDB-GENE-040219-9 NM_212813 403132 Dr.89442
tlr5a toll-like receptor 5a ZDB-GENE-040219-14 AY389449 403138 Dr.89423
tlr5b toll-like receptor 5b ZDB-GENE-040219-15 NM_001130595 403139 Dr.89707
tnfa tumor necrosis factor a (TNF superfamily, member 2) ZDB-GENE-050317-1 NM_212859 405785 Dr.89727
traf6 TNF receptor-associated factor 6 ZDB-GENE-030131-5735 NM_001044752 554561 Dr.74618
Reference genes     
bactin1 beta-actin 1 ZDB-GENE-000329-1 NM_131031 57934 Dr.35143
ppial peptidylprolyl isomerase A, like (cyclophilin) ZDB-GENE-030131-7459 NM_199957 335519 Dr.78109
rplp0 acidic ribosomal protein (ARP) ZDB-GENE-000629-1 NM_131580 58101 Dr.55617
  1. 1 Chemokine genes ccl-c5a, ccl-c24i, cxcl-c5c, cxcl-c1c, and cxcl-c13d are named by the chromosome on which they are located according to Nomiyama et al. [21]. All other gene names are according to ZFIN (http://www.zfin.org).
  2. 2 UniGene IDs are according to Build #122.