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Table 1 Target genes of the zebrafish immune response RT-MLPA assay

From: Rapid screening of innate immune gene expression in zebrafish using reverse transcription - multiplex ligation-dependent probe amplification

Gene name1

Description

ZFIN ID

GenBank Accession

Entrez Gene ID

UniGene ID2

Immune response genes

    

ccl20a

chemokine ccl-c24a (si:dkey-150o13.1, ccl20)

ZDB-GENE-091204-95

NM_001113595

563152

Dr.133624

ccl-c24i

transcribed locus, weakly similar to NP_001108573.1 chemokine CCL-Cub

-

CN021049

-

Dr.125570

ccl-c5a

chemokine ccl-c5a (si:ch211-89f7.4)

ZDB-GENE-060526-181

NM_001082906

794891

Dr.133987

chia.6

chitinase, acidic.6

ZDB-GENE-030131-1140

NM_199603

322420

Dr.77223

cxc46

cxc chemokine 46

ZDB-GENE-090303-1

XM_002664187

100321314

Dr.117585

cxcl-c13d

chemokine cxcl-c13d

-

NM_001113651

100003911

Dr.92011

cxcl-c1c

chemokine cxcl-c1c (si:ch73-6k14.1)

ZDB-GENE-090313-165

NM_001115060

795785

Dr.113696

cxcl-c5c

chemokine cxcl-c5c

-

NM_001115055

567537

Dr.111760

cxcr3.2

chemokine (C-X-C motif), receptor 3.2

ZDB-GENE-041114-186

NM_001007314

791973

Dr.82754

cxcr4b

chemokine (C-X-C motif), receptor 4b

ZDB-GENE-010614-1

NM_131834

114447

Dr.75485

fkbp5

FK506 binding protein 5

ZDB-GENE-030616-630

NM_213149

368924

Dr.78793

fos

v-fos FBJ murine osteosarcoma viral oncogene homolog

ZDB-GENE-031222-4

NM_205569

394198

Dr.12986

ifnphi1

interferon phi 1

ZDB-GENE-030721-3

NM_207640

360134

Dr.85981

il10

interleukin 10

ZDB-GENE-051111-1

NM_001020785

553957

Dr.135567

il1b

interleukin 1, beta

ZDB-GENE-040702-2

NM_212844

405770

Dr.30443

il8

interleukin 8

ZDB-GENE-081104-317

CT826376

100002946

Dr.112992

jun

v-jun sarcoma virus 17 oncogene homolog (c-Jun)

ZDB-GENE-030131-7859

NM_199987

335916

Dr.1064

junb

jun B proto-oncogene

ZDB-GENE-040426-2172

NM_213556

407086

Dr.10326

mmp9

matrix metalloproteinase 9

ZDB-GENE-040426-2132

NM_213123

406397

Dr.76275

mpx

myeloid-specific peroxidase

ZDB-GENE-030131-9460

NM_212779

337514

Dr.75725

myd88

myeloid differentiation primary response gene (88)

ZDB-GENE-040219-3

NM_212814

403145

Dr.134592

nfkb2

nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, p49/p100

ZDB-GENE-030131-6701

NM_001001840

415100

Dr.117553

rel

v-rel reticuloendotheliosis viral oncogene homolog

ZDB-GENE-040718-255

NM_001001841

415101

Dr.86023

rela

v-rel reticuloendotheliosis viral oncogene homolog A

ZDB-GENE-040825-4

NM_001001839

415099

Dr.84126

sarm1

sterile alpha and TIR motif containing 1

ZDB-GENE-040219-1

NM_001130596

403143

Dr.84799

spi1

spleen focus forming virus (SFFV) proviral integration oncogene spi1 (pu.1)

ZDB-GENE-980526-164

NM_198062

30117

Dr.34508

ticam1

toll-like receptor adaptor molecule 1 (ticam1, trif)

ZDB-GENE-040219-2

NM_001044759

403147

Dr.82215

tirap

toll-interleukin 1 receptor (TIR) domain containing adaptor protein (tirap, mal)

ZDB-GENE-040219-4

AL916644

403148

Dr.87438

tlr1

toll-like receptor 1

ZDB-GENE-040220-1

NM_001130593

403127

Dr.89709

tlr4bb

toll-like receptor 4b, duplicate b

ZDB-GENE-040219-9

NM_212813

403132

Dr.89442

tlr5a

toll-like receptor 5a

ZDB-GENE-040219-14

AY389449

403138

Dr.89423

tlr5b

toll-like receptor 5b

ZDB-GENE-040219-15

NM_001130595

403139

Dr.89707

tnfa

tumor necrosis factor a (TNF superfamily, member 2)

ZDB-GENE-050317-1

NM_212859

405785

Dr.89727

traf6

TNF receptor-associated factor 6

ZDB-GENE-030131-5735

NM_001044752

554561

Dr.74618

Reference genes

    

bactin1

beta-actin 1

ZDB-GENE-000329-1

NM_131031

57934

Dr.35143

ppial

peptidylprolyl isomerase A, like (cyclophilin)

ZDB-GENE-030131-7459

NM_199957

335519

Dr.78109

rplp0

acidic ribosomal protein (ARP)

ZDB-GENE-000629-1

NM_131580

58101

Dr.55617

  1. 1 Chemokine genes ccl-c5a, ccl-c24i, cxcl-c5c, cxcl-c1c, and cxcl-c13d are named by the chromosome on which they are located according to Nomiyama et al. [21]. All other gene names are according to ZFIN (http://www.zfin.org).
  2. 2 UniGene IDs are according to Build #122.