TFRC and ACTB as the best reference genes to quantify Urokinase Plasminogen Activator in breast cancer

BMC Research Notes

Table 2 Primer probe sets.

Target	Accession No.	Sequence	Melting TM	efficiency
GAPDH	NM_001002	F GAAGGTGAAGGTCGGAGTC	61.3	94
		R GGGTGGAATCATATTGGAACA	63.2
		P ATTTGGTCGTATTGGGCGCCTGGT	74.9
TFRC	NM_003234	F ACCGGCACCATCAAGCT	64.5	94
		R TGATCACGCCAGACTTTGC	65.2
		P TGAAAATTCATATGTCCCTCGTGAGGCT	72.1
RPLP0	NM_001002	F CGGACGAGGATATGGGATTTG	67.2	89
		R AGAAGTAAGCCTTTATTTCCTTGTTT	64.7
		P TCACCAAAAAGCAACCAACTTAGCCAGT	72
GUSB	NM_000181	F GCGTTCCTTTTGCGAGGAGA	68.6	74
		R GGTGGTATCAGTCTTGCTCAA	64.7
		P ACCAGGTATCCCCACTCAGTAGCCAAG	72
HPRT1	NM_000194	F TGGACTAATTATGGACAGGACTGAA	64.4	103
		R GTAATCCAGCAGGTCAGCAA	62.8
		P CTTGCTCGAGATGTGATGAAGGAGATGG	73.7
UPA	NM_002658	F AGGGCAGCACTGTGAAATAGATAAGT	65.7	97
		R CATGGTACGTTTGCTGAAGGA	64.8
		P TTACCGAGGAAAGGCCAGCACTGACA	75.3
ACTB	NM_001101	F CAGCAGATGTGGATCAGCAAG	65.9	95
		R GCATTTGCGGTGGACGAT	67.1
		P AGGAGTATGACGAGTCCGGCCCC	73.8

ISSN: 1756-0500