| |
Concentration
|
TNF-α (pg/ml)
|
IL-12 (pg/ml)
|
|---|
|
36 h cultured LC
|
-
|
21.8 ± 1.4
|
9.4 ± 0.5
|
|
Prednisone
|
10-6 M
|
10.8 ± 2.0*
|
2.9 ± 0.7**
|
| |
10-8 M
|
9.5 ± 0.9*
|
4.0 ± 1.1**
|
|
Thalidomide
|
10-6 M
|
7.8 ± 1.7*
|
4.8 ± 0.5**
|
| |
10-8 M
|
4.4 ± 3.8**
|
3.5 ± 1.8**
|
|
Cyclosporine A
|
10-6 M
|
5.8 ± 0.4**
|
8.7 ± 0.4
|
| |
10-8 M
|
7.6 ± 0.5**
|
8.5 ± 0.6
|
|
Amitriptyline
|
10-6 M
|
9.7 ± 2.2*
|
6.4 ± 0.3*
|
| |
10-8 M
|
12.2 ± 0.6*
|
7.4 ± 2.7
|
- aPurified LC were cultured for 36 h in the presence or absence of 10-6 M or 10-8 M prednisone, thalidomide, cyclosporine A, or amitriptyline. TNF-α and IL-12 levels in the culture supernatant were quantified by ELISA. All results are shown as mean ± SD for three independent experiments. *p < 0.05 vs. control. **p < 0.01 vs. control.
