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Table 2 In vitro effects of two different concentrations of immunomodulatory drugs on TNF-α and IL-12 production by LCa

From: Effects of immunomodulatory drugs on TNF-α and IL-12 production by purified epidermal langerhans cells and peritoneal macrophages

  Concentration TNF-α (pg/ml) IL-12 (pg/ml)
36 h cultured LC - 21.8 ± 1.4 9.4 ± 0.5
Prednisone 10-6 M 10.8 ± 2.0* 2.9 ± 0.7**
  10-8 M 9.5 ± 0.9* 4.0 ± 1.1**
Thalidomide 10-6 M 7.8 ± 1.7* 4.8 ± 0.5**
  10-8 M 4.4 ± 3.8** 3.5 ± 1.8**
Cyclosporine A 10-6 M 5.8 ± 0.4** 8.7 ± 0.4
  10-8 M 7.6 ± 0.5** 8.5 ± 0.6
Amitriptyline 10-6 M 9.7 ± 2.2* 6.4 ± 0.3*
  10-8 M 12.2 ± 0.6* 7.4 ± 2.7
  1. aPurified LC were cultured for 36 h in the presence or absence of 10-6 M or 10-8 M prednisone, thalidomide, cyclosporine A, or amitriptyline. TNF-α and IL-12 levels in the culture supernatant were quantified by ELISA. All results are shown as mean ± SD for three independent experiments. *p < 0.05 vs. control. **p < 0.01 vs. control.