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Figure 1 | BMC Research Notes

Figure 1

From: An improved PCR strategy for fast screening of specific and random integrations in rAAV-mediated gene targeted cell clones

Figure 1

Illustration of the targeting construct and screening PCRs. Illustration of rAAV-mediated gene targeting by homologous recombination (A), and random gene integration of the rAAV genome by non-homologous end joining (B). Primers for screening PCRs of gene targeting and random integrations of the rAAV genome are indicated by arrows. ITR: inverted terminal repeat sequences; HR: homologous recombination; LHA: left homology arm; RHA: right homology arm; DSB locus: double-strand break locus; NHEJ: non-homologous end joining; EM7: bacterial EM7 promoter.

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BMC Research Notes

ISSN: 1756-0500

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