Figure 9

Sequence analysis of important amino acid residues in less conservative regions of DGAT1s. (A) The boxed S residue is mutated in Tropaeolum majus DGAT1 corresponding to S197, (B) The boxed P residue is mutated in Tropaeolum majus DGAT1 corresponding to P216. Multiple sequence alignment was performed using 55 full-length DGAT1 protein sequences from 45 organisms (listed in Table 1). Color code and related information are described briefly in Figure 2 legend and with details in "Methods" section. The amino acid residues studied by mutagenesis and the corresponding conserved residues in other organisms are boxed within the sequence alignment.