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Table 1 Candidate reference genes evaluated in this study

From: Selection of suitable reference genes for quantitative real-time polymerase chain reaction in human meningiomas and arachnoidea

Gene Symbol Gene Name Genbank Acession No. TaqMan®Assay ID Amplicon
length
18S Eukaryotic 18S rRNA X03205.1 (mRNA) Hs99999901_s1 187
ABL1 v-abl Abelson murine leukemia oncogene homolog 1 NM_005157.3 + NM_007313.2 Hs00245445_m1 91
ACTB Actin, Beta, cytoplasmic NM_001101.3 Hs99999903_m1 171
B2M Beta-2-microglobulin NM_004048.2 Hs99999907_m1 75
CASC3 cancer susceptibility candidate 3 NM_007359.4 Hs00201226_m1 67
CDKN1A cyclin-dependent kinase inhibitor 1A (p21, Cip1) NM_078467.1 + NM_000389.3 Hs00355782_m1 66
CDKN1B cyclin-dependent kinase inhibitor 1B (p27, Kip1) NM_004064.3 Hs00153277_m1 71
EIF2B1 eukaryotic translation initiation factor 2B, subunit 1 alpha, 26 kDa NM_001414.3 Hs00426752_m1 75
ELF1 E74-like factor 1/ets domain transcription factor) NM_172373.3 + NM_001145353.1 Hs00152844_m1 76
GADD45A growth arrest and DNA-damage-inducible, alpha NM_001924.2 Hs00169255_m1 123
GAPDH Glyceraldehyde-3-phosphate dehydrogenase NM_002046.3 Hs99999905_m1 122
GUSB Glucuronidase, Beta NM_000181.3 Hs99999908_m1 81
HMBS Hydromethylbilane synthase NM_000190.3 Hs00609297_m1 64
HPRT1 Hypoxanthine guanine phospho- ribosyl transferase 1 NM_000194.2 Hs99999909_m1 100
IPO8 Improtin 8 NM_006390.2 Hs00183553_m1 71
MRPL19 mitochondrial ribosomal protein L19 NM_014763.3 Hs00608519_m1 72
MT-ATP6 mitochondrially encoded ATP synthase 6 NC_001807.ATP6.0 Hs02596862_g1 150
PES1 pescadillo homolog 1, containing BRCT domain (zebrafish) NM_014303.2 Hs00362795_g1 56
PGK1 Phosphoglycerate kinase 1 NM_000291.3 Hs99999906_m1 75
POLR2A Polymerase (RNA) II (DNA directed) polypeptide A, 220 kDa NM_000937.3 Hs00172181_m1 61
POP4 processing of precursor 4, ribonuclease P/MRP subunit NM_006627.2 Hs00198357_m1 68
PPIA Peptidylprolyl Isomerase A NM_021130.3 Hs99999904_m1 98
PSMC4 proteasome (prosome, macropain) 26S subunit, ATPase, 4 NM_153001.1 + NM_006503.2 Hs00197826_m1 83
PUM1 pumilio homolog 1 (Drosophila) NM_001020658.1 + NM_014676.2 Hs00206469_m1 89
RPL30 ribosomal protein L30 NM_000989.2 Hs00265497_m1 149
RPL37A ribosomal protein L37A NM_000998.4 Hs01102345_m1 125
RPLP0 Ribosomal protein, large, P0 NM_053275.3 + NM_001002.3 Hs99999902_m1 105
RPS17 ribosomal protein S17 NM_001021.3 Hs00734303_g1 93
TBP TATA binding box protein NM_008907 Hs99999910_m1 127
TFRC Transferrin receptor NM_001128148.1 + NM_003234.2 Hs99999911_m1 105
UBC Ubiquitin C NM_021009.4 Hs00824723_m1 71
YWHAZ Tyrosine 3-monooxygenase NM_003406.3 Hs00237047_m1 70
  1. Thickly printed reference genes were further evaluated. TaqMan® Assay ID ending with "_m" indicates an assay whose probe spans an exon junction and will not detect genomic DNA. "_g" indicates an assay that may detect genomic DNA. The assay primers and probe may also be within a single exon. "_s" indicates an assay whose primers and probes are designed within a single exon, such assays will, by definition detect genomic DNA. Additional information for each TaqMan® Assay is available from Applied Biosystems.