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Table 1 Yield and purity of DNA and RNA preparations from 0,8 mm cortex punches using commonly employed protocols, commercially available kits and the presented simultaneous DNA/RNA extraction method

From: Simultaneous DNA and RNA isolation from brain punches for epigenetics

Method DNA   Total yield [ng] 260/280 260/230
(n = 6) Qiagen Dneasy 360 ± 96 1,8 1,35
  CTAB 520 ± 87 1,8 1,5
  SDS/Prot K 850 ± 195 2 1,4
  Gentra Puregene 360 ± 96 2 1,5
Method RNA     
(n = 6) MN Nucleospin II 366 ± 75 2,1 0,2
  Trizol 690 ± 262 1,7 0,6
  Chomczynski 838 ± 321 1,9 1,4
Simultaneous DNA/RNA     
(n = 9) DNA 765 ± 163 1,9 1,3
  RNA 330 ± 54 1,9 1,2