Table 1 Genes and primers used for qRT-PCR analysis
Gene Abbreviation | Arachis species | GenBank ID | Gene description | Primer sequence Forward/ Reverse | Amplicon size (bp) | PCR efficiency (%) | Regression coefficient R2 |
|---|---|---|---|---|---|---|---|
60S | A. stenosperma | EH042095.1 | 60S ribosomal protein L10 | TGGAGTGAGAGGTGCATTTG/ TCTTTTGACGACCAGGGAAC | 155 | 99.872 | 0.994 |
ACT1 | A. magna | Not available | Actin depolymerizing factor-like protein | TGGTCTCGGTTTCCTGAGTT/ AATACCACTCCAAAGCAAACG | 114 | 98.330 | 1.000 |
ACT2 | A. hypogaea | GO326795.1 | Actin | GAGCTGAAAGATTCCGATGC/ GCAATGCCTGGGAACATAGT | 178 | 108.360 | 0.994 |
EFA | A. stenosperma | EH046450.1 | Chloroplast elongation factor tub | CGATGTCACTGGCAAGGTTA/ TAGCGAACCTCATTCCCTGT | 137 | 101.936 | 1.000 |
GAPDH | A. magna | Not available | Glyceraldehyde-3- phosphate dehydrogenase | CAACAACGGAGACATCAACG/ ATCACTGCCACCCAGAAAAC | 190 | 91.802 | 0.958 |
MAN | A. stenosperma | EH048114.1 | Mannose/glucose-binding lectin | ATTAAATCCGCTGCAACCAC/ AATCCAACCATACCCCATTC | 185 | 92.192 | 1.000 |
PRO | A. stenosperma | EH047960.1 | Proline-rich protein precursor | GCACCCAATTGAAAAACCAC/ GAGGGTACTTGCCATGAGGA | 185 | 90.180 | 1.000 |
TUB | A. stenosperma | EH047237.1 | Beta-tubulin | AGTCAGGTGCGGGTAACAAC/ CCAGTACCACCTCCCAAAGA | 151 | 97.668 | 1.000 |
UBI1 | A. stenosperma | EH047293.1 | Polyubiquitin | TCTTGTCCTCCGTCTTAGGG/ AGCAAGGGTCCTTCCATCTT | 196 | 99.997 | 0.999 |
UBI2* | A. hypogaea | HO115753.1 | Ubiquitin/ribosomal protein S27a | AAGCCGAAGAAGATCAAGCAC/ GGTTAGCCATGAAGGTTCCAG | 145 | 99.218 | 0.999 |