Commonly differentially regulated genes in unstressed aak-2 and stressed aak-2 and transcriptional changes specific to unstressed aak-2 mutants. (A) Venn diagram showing numbers of commonly up or (B) down-regulated genes with statistically significant gene expression level changes in stressed wild type, stressed aak-2 mutants, and unstressed aak-2 mutants (overlapping regions) as well as numbers of genes up or down-regulated exclusively in one library (non-overlapping regions). Notably, there is a relatively large subset of genes up (137) or down-regulated (92) significantly in unstressed aak-2 mutants, but either insignificantly regulated or significantly regulated in opposite direction in stressed wild type and stressed aak-2 mutants. (C) Genes significantly differentially expressed in unstressed aak-2 mutants relative to wild type, but insignificantly regulated or significantly changed in the opposite direction in stressed wild type or stressed aak-2 mutants. The genes are sorted by the expression level changes (log2 ratio) in unstressed aak-2 mutants, and some interesting genes are labelled such as vitellogenins (vit) and major sperm proteins (msp). For instance, the msp genes are highly down-regulated in unstressed aak-2 mutants as indicated by the red dots, but slightly up regulated on average in stressed wild type and stressed aak-2 mutants as shown by the blue and green dots respectively.