Table 2 Primer sequences, exon locations, amplicon size, and optimal melting temperature of canine candidate reference genes.
From: Leukocyte count affects expression of reference genes in canine whole blood samples
Gene | Forward 5' →3'* | Exon(s) | Reverse 5' → 3' | Exon(s) | Product length (bp) | Tm(°C) |
|---|---|---|---|---|---|---|
RPS19 | CCTTCCTCAAAAA/GTCTGGG | 2/3 | GTTCTCATCGTAGGGAGCAAG | 3 | 95 | 61.0 |
RPL8 | CCATGAAT/CCTGTGGAGC | 4/5 | GTAGAGGGTTTGCCGATG | 5 | 64 | 55.0 |
RPS5 | TCACTGGTGAG/AACCCCCT | 2/3 | CCTGATTCACACGGCGTAG | 3 | 141 | 62.5 |
GUSB | AGACGCTTCCAA/GTACCCC | 3/4 | AGGTGTGGTGTAGAGGAGCAC | 4 | 103 | 62.0 |
B2M | TCCTCATCCTCCTCGCT | 1 | TTCTCTGCTGGGTGTCG | 2 | 85†| 61.2 |
HNRNPH | CTCACTATGATCCACCACG | 5 | TAGCCTCCATAAC/CTCCAC | 5/6 | 151 | 61.2 |
HPRT | AG/CTTGCTGGTGAAAAGGAC | 5/6 | TTATAGTCAAGGGCATATCC | 7 | 114‡ | 56.0 |
GAPDH | TGTCCCCACCCCCAATGTATC | 2 | CTCCGATGCCTGCTTCACTACCTT | 2 | 100 | 58.0 |
SRPR | GCTTCAGGATCTGGACTGC | 5/6 | GTTCCCTTGGTAGCACTGG | 6 | 81 | 61.2 |