Leukocyte count affects expression of reference genes in canine whole blood samples

BMC Research Notes

Table 3 Relation of Cq and white blood cell count.

Gene	Cq change for 10 fold increase in WBC count	p	WBC within reference range		WBC > 30*10⁹/l		Difference	p
			Mean Cq	SD	Mean Cq	SD
RPS19	0.92	0	17.35	0.82	17.90	1.31	0.56	0.002
RPL8	0.52	0.006	19.20	0.67	19.52	1.14	0.32	0.026
RPS5	1.06	0	18.30	0.86	19.01	1.61	0.71	0.0009
GUSB	- 0.07	0.75	21.09	0.97	21.08	1.47	-0.01	0.93
B2M	1.28	0	15.85	0.86	16.58	1.74	0.73	0.0006
HNRNPH	- 0.09	0.68	22.40	0.86	22.20	1.02	-0.20	0.19
HPRT	- 0.87	0	21.14	0.93	20.71	1.03	-0.43	0.01
GAPDH	- 0.51	0.023	14.87	0.97	14.80	0.96	-0.07	0.38
SRPR	0.10	0.70	18.30	1.09	19.01	1.51	0.03	0.91

• WBC = White Blood Cell Count
Change in Cq is given for a ten fold increase in WBC count, mean Cq and SD for dogs with WBC count in the reference range and for dogs with a leukocytosis with a WBC > 30*10⁹/l.

ISSN: 1756-0500