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Table 3 Relation of Cq and white blood cell count.

From: Leukocyte count affects expression of reference genes in canine whole blood samples

Gene Cq change for 10 fold increase in WBC count p WBC within reference range WBC > 30*109/l Difference p
    Mean Cq SD Mean Cq SD   
RPS19 0.92 0 17.35 0.82 17.90 1.31 0.56 0.002
RPL8 0.52 0.006 19.20 0.67 19.52 1.14 0.32 0.026
RPS5 1.06 0 18.30 0.86 19.01 1.61 0.71 0.0009
GUSB - 0.07 0.75 21.09 0.97 21.08 1.47 -0.01 0.93
B2M 1.28 0 15.85 0.86 16.58 1.74 0.73 0.0006
HNRNPH - 0.09 0.68 22.40 0.86 22.20 1.02 -0.20 0.19
HPRT - 0.87 0 21.14 0.93 20.71 1.03 -0.43 0.01
GAPDH - 0.51 0.023 14.87 0.97 14.80 0.96 -0.07 0.38
SRPR 0.10 0.70 18.30 1.09 19.01 1.51 0.03 0.91
  1. • WBC = White Blood Cell Count
  2. Change in Cq is given for a ten fold increase in WBC count, mean Cq and SD for dogs with WBC count in the reference range and for dogs with a leukocytosis with a WBC > 30*109/l.