Figure 3
From: Selection of reference genes for qPCR in hairy root cultures of peanut
Comparison of integrity and yields of RNA between lyophilized and frozen peanut hairy roots. (A) RNA integrity was compared between lyophilized and frozen material. The two ribosomal subunits (28S and 18S) are indicators of RNA integrity on agarose gel electrophoresis. Peanut hairy roots elicited with NaOAc and non-elicited (control) were also compared. Numbers 1 to 12 represent biological replicates. (B) RNA yields were compared between these two conditions. For lyophilized tissue, RNA yields were expressed based on fresh weight (FW). Bars represent the average of three biological replicates and error bars represent the standard deviation. Total RNA from 9-day old root cultures was extracted with TRIzol® and quantified spectrophotometrically as described in Methods section.