Table 2 The consequence of 454 antibody sequencing errors affecting the functionality of antibodies as determined by IMGT/HighV-QUEST using sequence data obtained from the two repeat 454 runs, 1 and 2.
From: 454 antibody sequencing - error characterization and correction
Productive | 65.8 (16.1)a | 68 (23)a |
|---|---|---|
Unproductive | 13.7 | 5.3 |
Stop codon | 3.7 | 3.1 |
No rearrangement | 0.6 | 0.5 |
Functionality | Run 1 (%) | Run 2 (%) |
Productive | 65.8 (16.1)a | 68 (23)a |