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Table 2 The consequence of 454 antibody sequencing errors affecting the functionality of antibodies as determined by IMGT/HighV-QUEST using sequence data obtained from the two repeat 454 runs, 1 and 2.

From: 454 antibody sequencing - error characterization and correction

Productive 65.8 (16.1)a 68 (23)a
Unproductive 13.7 5.3
Stop codon 3.7 3.1
No rearrangement 0.6 0.5
Functionality Run 1 (%) Run 2 (%)
Productive 65.8 (16.1)a 68 (23)a
  1. aThe percentage value of additional productive sequences recovered from the pool of unproductive sequences by correcting the errors due to mainly insertions.