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Table 1 Comparison of the 454 assemblies of barcoded BACs with their Sanger reference sequences.

From: Sequencing of BAC pools by different next generation sequencing platforms and strategies

BAC Data sets1 sequence depth average read length (bp) L50 (bp) L80 (bp) misass2 gaps total gap size (bp) penalties3
  bcFLX 27 224 52,352 52,352 0 1 50 1
184G09 120,562 bp bcTi 56 256 121,630 121,630 0 0 0 0
  bcTids 27 256 120,569 120,569 0 0 0 0
  bcFLX 15 225 11,912 3,586 6 9 490 21
259I16 124,050 bp bcTi 25 253 68,888 14,394 2 3 177 7
  bcTids 15 253 24,258 10,367 6 5 355 17
  bcFLX 26 223 52,601 11,098 6 5 199 17
631P08 101,158 bp bcTi 66 252 25,788 17,610 2 2 77 6
  bcTids 26 252 52,257 17,582 1 1 14 3
  bcFLX 26 219 16,866 3,203 17 4 392 38
711N16 112,178 bp bcTi 41 292 21,923 3,860 5 3 30 13
  bcTids 26 292 21,921 3,859 5 3 25 13
  bcFLX 23 223 33,433 17,560 29 19 1,131 77
all bcTi 47 263 59,557 39,374 9 8 284 26
  bcTids 23 263 54,751 38,094 12 9 394 33
  1. 1 bcFLX: barcoded FLX; bcTi: barcoded Titanium; bcTids: barcoded Titanium, down sampled to the same sequence depth as of bcFLX.
  2. 2 misassemblies
  3. 3 To quantify the assembly quality, penalties of 2 per misassembly and 1 per gap were given.