Sequencing of BAC pools by different next generation sequencing platforms and strategies

BMC Research Notes

Table 4 Comparison of the 96 barcoded BAC 454 assemblies without and with scaffolding by Illumina MPs.

contigs and scaffolds	without scaffolding	with scaffolding	Fold change
Average [bp]	7,546	11,161	1.5
Maximum [bp]	89,835	114,443	1.3
L50¹[bp]	21,694	53,258	2.5
L80 [bp]	7,392	22,695	3.1
L90 [bp]	3,784	7,644	2.0
N50²	157	67	0.4
N80	418	161	0.4
N90	616	244	0.4

¹ Length of the contig such that using equal or longer contigs produces 50% of the overall assembly length;
² Number of largest contigs such that their bases produce 50% of the overall assembly length.

ISSN: 1756-0500