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Figure 2 | BMC Research Notes

Figure 2

From: Double conditional human embryonic kidney cell line based on FLP and ΦC31 mediated transgene integration

Figure 2

Independent integration and induction of fluorescent reporters. Cells of the cell line 19-3-1 have the Shld1 inducible DD-EYFP protein integrated into the attP docking site and the doxycycline inducible DsRed protein integrated into the FRT docking site. Cells were cultured in six well plates and incubated with 1 μg/ml doxycycline or 1 μM Shld1 as indicated. After 24 h fluorescence was observed and documented using a Leica MZ-FL III with appropriate filter sets as indicated.

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