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Figure 3 | BMC Research Notes

Figure 3

From: Double conditional human embryonic kidney cell line based on FLP and ΦC31 mediated transgene integration

Figure 3

Quantification of reporter activation by western blot. Lysates of cell line 16-1-3 (A) or 19-3-1 (B) were probed by western blots using an anti-DsRed antibody (left panels) or an anti-GFP antibody (right panels). Cell lysates were diluted as indicated. Note that the GFP antibody recognizes EYFP as well as ECFP. 1 μg/ml doxycycline or 1 μM Shld1 was added as indicated.

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