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Table 4 Gene ranking and consensus ranking using SYBR green super mixtures from two different vendors, Bio-Rad and Applied Biosystems

From: Reference gene validation for qPCR in rat carotid body during postnatal development

  Gene and consensus ranking for group N1+N14 (n = 16) using Bio-Rad SYBR
  Bio-Rad SYBR
Rank Position geNorm M-value NormFinder Variability BestKeeper Coeff. of corr. [r] Consensus
1 TBP 0.26 GAPDH 0.01 PPIA 0.89 PPIA
2 RPII 0.26 PPIA 0.011 Actin 0.84 GAPDH
3 PPIA 0.27 Actin 0.014 GAPDH 0.83 Actin
4 Actin 0.34 TBP 0.015 18s 0.73 TBP
5 GAPDH 0.38 RPII 0.016 RPII 0.72 RPII
6 18s 0.40 18s 0.018 TBP 0.70 18s
  Gene and consensus ranking for group N1 + N14 (n = 16) using Applied Biosystems SYBR
  Applied Biosystems SYBR
Rank Position geNorm M-value NormFinder Variability BestKeeper Coeff. of corr. [r] Consensus
1 PPIA 0.21 PPIA 0.008 PPIA 0.99 PPIA
2 GAPDH 0.21 GAPDH 0.013 GAPDH 0.99 GAPDH
3 Actin 0.25 Actin 0.013 Actin 0.99 Actin
4 RPII 0.32 RPII 0.014 RPII 0.98 RPII
5 TBP 0.34 TBP 0.016 TBP 0.98 TBP
6 18s 0.39 18s 0.024 18s 0.97 18s
  1. The reference genes are ranked for N1+N14 group by geNorm, Normfinder and BestKeeper and the consensus ranking determined by rank aggregation. (n) = number of independent determinations.