Age-related changes in relative expression stability of commonly used housekeeping genes in selected porcine tissues

Table 1 Selected candidate reference genes, primers, and PCR reactions efficiencies

Gene name	GeneBank accession number	Primer sequence (forward/reverse)	Amplicon length (bp)	Amplification efficiency (%)	R²	Average Ct of cDNA
						1 Day	2 months	5 months
B2M	NM_213978.1	ACTTTTCACACCGCTCCAGT CGGATGGAACCCAGATACAT	180	86.83	0.999	20.23	19.24	20.63
BLM	NM_001123084.1	TCCTCACCTTCTGCATTTCC GTGGTGGCTGAGAATCCTGT	152	95.94	0.995	25.29	24.12	24.89
GAPDH	AF017079.1	ACCCAGAAGACTGTGGATGG ACGCCTGCTTCACCACCTTC	247	95.95	0.991	26.82	26.22	26.29
HPRT1	NM_001032376.2	AACCTTGCTTTCCTTGGTCA TCAAGGGCATAGCCTACCAC	150	81.88	0.997	22.27	21.28	22.29
PPIA	NM_214353.1	CACAAACGGTTCCCAGTTTT TGTCCACAGTCAGCAATGGT	171	82.96	0.995	16.82	16.31	17.61
RPL4	DQ845176.1	AGGAGGCTGTTCTGCTTCTG TCCAGGGATGTTTCTGAAGG	185	91.07	0.995	16.65	16.80	17.32
SDHA	DQ178128.1	AGAGCCTCAAGTTCGGGAAG CAGGAGATCCAAGGCAAAAT	149	86.41	0.989	20.55	20.64	22.34
TBP	DQ178129.1	ACGTTCGGTTTAGGTTGCAG GCAGCACAGTACGAGCAACT	118	99.59	0.995	24.44	23.92	24.31
YWHAZ	DQ178130.1	ATTGGGTCTGGCCCTTAACT GCGTGCTGTCTTTGTATGACTC	146	93.83	0.997	20.35	19.64	19.92

ISSN: 1756-0500