Differential production of proteins in M. haemolytica SH1217 in response to NaNO
supplementation in the media, shown by ISO-DALT 2D electrophoresis. A 900 μg of total protein preparations from SH1217 grown with or without NaNO3 supplementation were separated by isoelectric focusing on a 17 cm IEF strip. The proteins were separated in the second dimension by SDS-PAGE and visualized by Colloidal Coomassie blue staining. Red circles note the spots which showed over 1.5 fold increase in their volume compared to the volume of the same spot in the other gel. These spots were identified by mass spectrometry, and are numbered according to Table 1. The spot used for normalization in statistical analysis is labeled 'N'.