Table 2 Antibodies used in the study
Antibody | Clone* | Antigen retrieval | Primary antibody incubation | Concentrations used |
|---|---|---|---|---|
anti-Smad4 | Clone B-8 | Pressure cooker for 20 min | 2 h at room temperature | 2 μg/ml (1:100) |
anti-Smad6 | Clone H-150 | Microwaving:3 Cycles 3 min +1 Cycle 1 min | Overnight at 4°C | 4 μg/ml (1:50) |
anti-Smad7 | Clone H-79 | Microwaving: 3 Cycles 3 min +1 Cycle 1 min | Overnight at 4°C | 4 μg/ml (1:50) |