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Table 2 Antibodies used in the study

From: A study of Smad4, Smad6 and Smad7 in Surgically Resected Samples of Pancreatic Ductal Adenocarcinoma and Their Correlation with Clinicopathological Parameters and Patient Survival

Antibody Clone* Antigen retrieval Primary antibody incubation Concentrations used
anti-Smad4 Clone B-8 Pressure cooker for 20 min 2 h at room temperature 2 μg/ml (1:100)
anti-Smad6 Clone H-150 Microwaving:3 Cycles 3 min +1 Cycle 1 min Overnight at 4°C 4 μg/ml (1:50)
anti-Smad7 Clone H-79 Microwaving: 3 Cycles 3 min +1 Cycle 1 min Overnight at 4°C 4 μg/ml (1:50)
  1. *All antibodies were obtained from Santa Cruz Biotechnology Inc., CA., USA.