Table 3 Primers designed for amplification and sequencing of HBV reverse transcriptase domain
Primer ID | Nucleotide sequences | Product size (bp) | Nucleotide positions | ||
|---|---|---|---|---|---|
HBV TAQ 1 | 5' GTG TCT GCG GCG TTT TATCA -3' | Sense | 97 | DNA quantification | 379-398 |
HBV TAQ 2 | 5' GAC AAA CGG GCA ACA TAC CTT-3' | Antisense | 476-456 | ||
MM HBRT5 | 5' - ATCCTGCTGCTATGCCT - 3' | Sense | 576 | polymerase Amplification | 410 - 426 |
MM HBRT6 | 5' - ACTTTCCAATCAATAGGCC - 3' | Antisense | 986 - 968 |