Figure 1
From: A modified agar pad method for mycobacterial live-cell imaging
![Figure 1](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2F1756-0500-4-73/MediaObjects/13104_2010_Article_830_Fig1_HTML.jpg)
Apparatus for live-cell imaging using the modified agar pad method. Cells are seeded onto the glass cover slip of a 35 mm glass-bottomed dish. Agar (+/- dye, antibiotic, inducer) is then added to the dish and the cells viewed from below using an inverted microscope. Supplementation of the agar can also be achieved mid-experiment by removal of the lid and application of the supplement directly to the cell surface of the agar which can diffuse through the agar pad to the cells.