Figure 1

Apparatus for live-cell imaging using the modified agar pad method. Cells are seeded onto the glass cover slip of a 35 mm glass-bottomed dish. Agar (+/- dye, antibiotic, inducer) is then added to the dish and the cells viewed from below using an inverted microscope. Supplementation of the agar can also be achieved mid-experiment by removal of the lid and application of the supplement directly to the cell surface of the agar which can diffuse through the agar pad to the cells.