Figure 4

Verification of Sp1 TFBSs targeting site-specific ChIP on tissue extracts. Kidney carcinoma samples were chosen for adaptation of the site-specific ChIP to tissue material. Tissue samples were formaldehyde-fixed and nuclei were isolated as described. Sp1 TFBSs and enzyme cutting sites information, enzyme digestion, immunoprecipitation and PCR analysis are as described in the legend to Figure 3, with exception of the uncleaved Kidney DNA from whole cell lysate (uc-Kidney, lanes 21, 24, 27, 30, 33). White arrows depict the bound Sp1 binding sites.